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BioVendor Instruments recombination human full length adiponectin
Circulating <t> adiponectin </t> levels and risk of nasopharyngeal carcinoma in retrospective and prospective cohorts

Circulating <t> adiponectin </t> levels and risk of nasopharyngeal carcinoma in retrospective and prospective cohorts

Recombination Human Full Length Adiponectin, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombination+human+full+length+adiponectin/Adiponectin+Globular+Human+E%2E+coli/pmc08843017-64-0-19
Average 94 stars, based on 10 article reviews

recombination human full length adiponectin - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway"

Article Title: Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway

Journal: Journal of Translational Medicine

doi: 10.1186/s12967-022-03283-0

Figure Legend Snippet: Circulating adiponectin levels and risk of nasopharyngeal carcinoma in retrospective and prospective cohorts

Techniques Used:

Adiponectin suppresses nasopharyngeal carcinoma growth. A , B 1 × 10 6 CNE-2 cells were injected subcutaneously into 5- to 6- week-old adiponectin-deficient nude mice, or the control nude mice ( n = 6 per group). Tumor growth were monitored by measuring the tumor volume for 10 days. Next, the mice were sacrificed, and tumors were collected, measured, weighed. C The plate colony assay was performed to determine colony-formation ability. CNE-2 and C666-1 cells were treated with various concentrations of adiponectin for 7 days. Graphs show the number of colonies. D EdU incorporation assay was performed to determine cell proliferation. CNE-2 and C666-1 cells were treated with various concentrations of adiponectin for 48 h. Bars: 50 μm. Graphs show the relative cell proliferation percentage. E , F CNE-2 and C666-1 cells were incubated with adiponectin (40 μg/mL) for 48 h. At the end of incubation, the cells were collected for FACS analysis. G Western blot analysis of p-AMPKα (T172), p-LKB1 and p-ERk1/2 in cultured CNE-2 and C666-1 cells after the treatment with adiponectin (40 μg/mL) for 30 min. Results are presented as mean ± SD of three independent experiments performed in triplicate. * P < 0.05, ** P < 0.01, *** P < 0.001, # P < 0.0001

Figure Legend Snippet: Adiponectin suppresses nasopharyngeal carcinoma growth. A , B 1 × 10 6 CNE-2 cells were injected subcutaneously into 5- to 6- week-old adiponectin-deficient nude mice, or the control nude mice ( n = 6 per group). Tumor growth were monitored by measuring the tumor volume for 10 days. Next, the mice were sacrificed, and tumors were collected, measured, weighed. C The plate colony assay was performed to determine colony-formation ability. CNE-2 and C666-1 cells were treated with various concentrations of adiponectin for 7 days. Graphs show the number of colonies. D EdU incorporation assay was performed to determine cell proliferation. CNE-2 and C666-1 cells were treated with various concentrations of adiponectin for 48 h. Bars: 50 μm. Graphs show the relative cell proliferation percentage. E , F CNE-2 and C666-1 cells were incubated with adiponectin (40 μg/mL) for 48 h. At the end of incubation, the cells were collected for FACS analysis. G Western blot analysis of p-AMPKα (T172), p-LKB1 and p-ERk1/2 in cultured CNE-2 and C666-1 cells after the treatment with adiponectin (40 μg/mL) for 30 min. Results are presented as mean ± SD of three independent experiments performed in triplicate. * P < 0.05, ** P < 0.01, *** P < 0.001, # P < 0.0001

Techniques Used: Injection, Colony Assay, Incubation, Western Blot, Cell Culture

Adiponectin suppresses proliferation of NPC cells via AMPK activation. A , B CNE-2 and C666-1 cells were pretreated with compound C (10 mM) followed by treatment with adiponectin (40 μg/mL) for 24 h. Cell cycle was then analyzed using flow cytometer. # P < 0.05 and ## P < 0.01 compared to cells treated with adiponectin but not ComC; ** P < 0.01 compared with cells treated without adiponectin and ComC. C CNE-2 and C666-1 cells viability was determined after treatment with or without adiponectin for 48 h in the presence or absence of ComC (10 μM). ** P < 0.01, *** P < 0.001. D CNE-2 and C666-1 cells were pretreated with compound C (10 mM), p-AMPKα (Thr172) protein level was then determined by Western blot analysis after the treatment with adiponectin (40 μg/mL) for 30 min; cyclin D1, p21, and p27 protein level was then determined by Western blot analysis after the cells were exposed to adiponectin (40 μg/mL) for 48 h. E Quantitative analysis of p-AMPKα level was performed by densitometric analysis. Results are presented as mean ± SD of three independent experiments performed in triplicate. ** P < 0.01, *** P < 0.001

Figure Legend Snippet: Adiponectin suppresses proliferation of NPC cells via AMPK activation. A , B CNE-2 and C666-1 cells were pretreated with compound C (10 mM) followed by treatment with adiponectin (40 μg/mL) for 24 h. Cell cycle was then analyzed using flow cytometer. # P < 0.05 and ## P < 0.01 compared to cells treated with adiponectin but not ComC; ** P < 0.01 compared with cells treated without adiponectin and ComC. C CNE-2 and C666-1 cells viability was determined after treatment with or without adiponectin for 48 h in the presence or absence of ComC (10 μM). ** P < 0.01, *** P < 0.001. D CNE-2 and C666-1 cells were pretreated with compound C (10 mM), p-AMPKα (Thr172) protein level was then determined by Western blot analysis after the treatment with adiponectin (40 μg/mL) for 30 min; cyclin D1, p21, and p27 protein level was then determined by Western blot analysis after the cells were exposed to adiponectin (40 μg/mL) for 48 h. E Quantitative analysis of p-AMPKα level was performed by densitometric analysis. Results are presented as mean ± SD of three independent experiments performed in triplicate. ** P < 0.01, *** P < 0.001

Techniques Used: Activation Assay, Flow Cytometry, Western Blot

AdipoR1 and AdipoR2 mediate the anti-proliferative effect of adiponectin in NPC cells. A , B Expression of AdipoR1 and AdipoR2 was determined by qRT-PCR and Western blot in NPC cell lines. C , D CNE-2 and C666-1 cells were transfected with 50 μM siRNAs of NC, AdipoR1, or AdipoR2. The relative amounts of each AdipoR1/R2 mRNA against β-actin were measured with qRT-PCR. E Effect of the knockdown of AdipoR1 and AdipoR2 expression on the cell viability of CNE-2 and C666-1 cells treated with or without 40 μg/mL adiponectin for 48 h. F CNE-2 and C666-1 cells were transfected with AdipoR1, AdipoR2 siRNA or NC siRNA and treated with 40 μg/mL adiponectin for 30 min. AMPKα and p-AMPKα (T172) protein levels were determined by Western blot analysis. Quantitative analysis of p-AMPKα level was performed by densitometric analysis and shown in the below part. Results are presented as mean ± SD of three independent experiments performed in triplicate. ** P < 0.01, *** P < 0.001

Figure Legend Snippet: AdipoR1 and AdipoR2 mediate the anti-proliferative effect of adiponectin in NPC cells. A , B Expression of AdipoR1 and AdipoR2 was determined by qRT-PCR and Western blot in NPC cell lines. C , D CNE-2 and C666-1 cells were transfected with 50 μM siRNAs of NC, AdipoR1, or AdipoR2. The relative amounts of each AdipoR1/R2 mRNA against β-actin were measured with qRT-PCR. E Effect of the knockdown of AdipoR1 and AdipoR2 expression on the cell viability of CNE-2 and C666-1 cells treated with or without 40 μg/mL adiponectin for 48 h. F CNE-2 and C666-1 cells were transfected with AdipoR1, AdipoR2 siRNA or NC siRNA and treated with 40 μg/mL adiponectin for 30 min. AMPKα and p-AMPKα (T172) protein levels were determined by Western blot analysis. Quantitative analysis of p-AMPKα level was performed by densitometric analysis and shown in the below part. Results are presented as mean ± SD of three independent experiments performed in triplicate. ** P < 0.01, *** P < 0.001

Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Transfection

Injection:

Article Title: Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway
Article Snippet: cell line was authenticated via deoxyribonucleic-acid profiling using short tandem repeat analysis. .. Recombination human full-length adiponectin was dissolved in deionized water to prepare a working stock solution of approximately 0.5 mg/mL (BioVendor, Brno, Czech Republic). .. AdipoRon was dissolved in DMSO to prepare a working stock solution of approximately 50 mM (Selleck Chem

Colony Assay:

Incubation:

Western Blot:

Cell Culture:

Activation Assay:

Flow Cytometry:

Expressing:

Quantitative RT-PCR:

Transfection:

Image Search Results

Journal: Journal of Translational Medicine

Article Title: Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway

doi: 10.1186/s12967-022-03283-0

Figure Lengend Snippet: Circulating adiponectin levels and risk of nasopharyngeal carcinoma in retrospective and prospective cohorts

Article Snippet: Recombination human full-length adiponectin was dissolved in deionized water to prepare a working stock solution of approximately 0.5 mg/mL (BioVendor, Brno, Czech Republic).

Techniques:

Journal: Journal of Translational Medicine

Article Title: Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway

doi: 10.1186/s12967-022-03283-0

Figure Lengend Snippet: Adiponectin suppresses nasopharyngeal carcinoma growth. A , B 1 × 10 6 CNE-2 cells were injected subcutaneously into 5- to 6- week-old adiponectin-deficient nude mice, or the control nude mice ( n = 6 per group). Tumor growth were monitored by measuring the tumor volume for 10 days. Next, the mice were sacrificed, and tumors were collected, measured, weighed. C The plate colony assay was performed to determine colony-formation ability. CNE-2 and C666-1 cells were treated with various concentrations of adiponectin for 7 days. Graphs show the number of colonies. D EdU incorporation assay was performed to determine cell proliferation. CNE-2 and C666-1 cells were treated with various concentrations of adiponectin for 48 h. Bars: 50 μm. Graphs show the relative cell proliferation percentage. E , F CNE-2 and C666-1 cells were incubated with adiponectin (40 μg/mL) for 48 h. At the end of incubation, the cells were collected for FACS analysis. G Western blot analysis of p-AMPKα (T172), p-LKB1 and p-ERk1/2 in cultured CNE-2 and C666-1 cells after the treatment with adiponectin (40 μg/mL) for 30 min. Results are presented as mean ± SD of three independent experiments performed in triplicate. * P < 0.05, ** P < 0.01, *** P < 0.001, # P < 0.0001

Article Snippet: Recombination human full-length adiponectin was dissolved in deionized water to prepare a working stock solution of approximately 0.5 mg/mL (BioVendor, Brno, Czech Republic).

Techniques: Injection, Colony Assay, Incubation, Western Blot, Cell Culture

Journal: Journal of Translational Medicine

Article Title: Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway

doi: 10.1186/s12967-022-03283-0

Figure Lengend Snippet: Adiponectin suppresses proliferation of NPC cells via AMPK activation. A , B CNE-2 and C666-1 cells were pretreated with compound C (10 mM) followed by treatment with adiponectin (40 μg/mL) for 24 h. Cell cycle was then analyzed using flow cytometer. # P < 0.05 and ## P < 0.01 compared to cells treated with adiponectin but not ComC; ** P < 0.01 compared with cells treated without adiponectin and ComC. C CNE-2 and C666-1 cells viability was determined after treatment with or without adiponectin for 48 h in the presence or absence of ComC (10 μM). ** P < 0.01, *** P < 0.001. D CNE-2 and C666-1 cells were pretreated with compound C (10 mM), p-AMPKα (Thr172) protein level was then determined by Western blot analysis after the treatment with adiponectin (40 μg/mL) for 30 min; cyclin D1, p21, and p27 protein level was then determined by Western blot analysis after the cells were exposed to adiponectin (40 μg/mL) for 48 h. E Quantitative analysis of p-AMPKα level was performed by densitometric analysis. Results are presented as mean ± SD of three independent experiments performed in triplicate. ** P < 0.01, *** P < 0.001

Article Snippet: Recombination human full-length adiponectin was dissolved in deionized water to prepare a working stock solution of approximately 0.5 mg/mL (BioVendor, Brno, Czech Republic).

Techniques: Activation Assay, Flow Cytometry, Western Blot

Journal: Journal of Translational Medicine

Article Title: Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway

doi: 10.1186/s12967-022-03283-0

Figure Lengend Snippet: AdipoR1 and AdipoR2 mediate the anti-proliferative effect of adiponectin in NPC cells. A , B Expression of AdipoR1 and AdipoR2 was determined by qRT-PCR and Western blot in NPC cell lines. C , D CNE-2 and C666-1 cells were transfected with 50 μM siRNAs of NC, AdipoR1, or AdipoR2. The relative amounts of each AdipoR1/R2 mRNA against β-actin were measured with qRT-PCR. E Effect of the knockdown of AdipoR1 and AdipoR2 expression on the cell viability of CNE-2 and C666-1 cells treated with or without 40 μg/mL adiponectin for 48 h. F CNE-2 and C666-1 cells were transfected with AdipoR1, AdipoR2 siRNA or NC siRNA and treated with 40 μg/mL adiponectin for 30 min. AMPKα and p-AMPKα (T172) protein levels were determined by Western blot analysis. Quantitative analysis of p-AMPKα level was performed by densitometric analysis and shown in the below part. Results are presented as mean ± SD of three independent experiments performed in triplicate. ** P < 0.01, *** P < 0.001

Article Snippet: Recombination human full-length adiponectin was dissolved in deionized water to prepare a working stock solution of approximately 0.5 mg/mL (BioVendor, Brno, Czech Republic).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection

Profiles of patients with MM enrolled in the present study.

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: Profiles of patients with MM enrolled in the present study.

Article Snippet: Recombinant human full-length adiponectin (PeproTech, Inc., Rocky Hill, NJ, USA) was dissolved in water at a concentration of 1.0 mg/ml and then diluted with 0.1% bovine serum albumin to a final concentration of 0.1 mg/ml.

Techniques: Control

Visfatin, leptin, and adiponectin levels in newly diagnosed patients with MM. * P<0.05 with comparisons shown by lines. MM, multiple myeloma.

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: Visfatin, leptin, and adiponectin levels in newly diagnosed patients with MM. * P<0.05 with comparisons shown by lines. MM, multiple myeloma.

Techniques:

Levels of visfatin, leptin and adiponectin in patients with different ISS phase multiple myeloma.

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: Levels of visfatin, leptin and adiponectin in patients with different ISS phase multiple myeloma.

Techniques:

Levels of visfatin, leptin and adiponectin in different multiple myeloma bone disease stages.

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: Levels of visfatin, leptin and adiponectin in different multiple myeloma bone disease stages.

Techniques:

Correlations of visfatin, leptin and adiponectin levels with indices of bone disease (OCN, CTX and PINP).

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: Correlations of visfatin, leptin and adiponectin levels with indices of bone disease (OCN, CTX and PINP).

Techniques:

Correlation between adiponectin, and either OCN or CTX levels in patients with multiple myeloma. (A) Serum level of adiponectin is positively correlated with OCN levels. (B) Serum level of adiponectin is negatively correlated with CTX levels. OCN, osteocalcin; CTX, carboxy-terminal cross-linking telopeptide of type I collagen.

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: Correlation between adiponectin, and either OCN or CTX levels in patients with multiple myeloma. (A) Serum level of adiponectin is positively correlated with OCN levels. (B) Serum level of adiponectin is negatively correlated with CTX levels. OCN, osteocalcin; CTX, carboxy-terminal cross-linking telopeptide of type I collagen.

Techniques:

Adiponectin inhibits osteoclast differentiation and maturation. TRAP staining was performed in (A) the control group and (B) the adipo-nectin-treated group (original magnification, ×100). (C) Quantified number of TRAP-positive multinucleated cells. * P<0.05 with comparisons shown by lines. TRAP, tartrate-resistant acid phosphatase.

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: Adiponectin inhibits osteoclast differentiation and maturation. TRAP staining was performed in (A) the control group and (B) the adipo-nectin-treated group (original magnification, ×100). (C) Quantified number of TRAP-positive multinucleated cells. * P<0.05 with comparisons shown by lines. TRAP, tartrate-resistant acid phosphatase.

Techniques: Staining, Control

mRNA levels of the osteoclast-specific factors RANKL, OSCAR, TRAP and Cathepsin K. Expression levels of mRNA are presented for the control and adiponectin-treated group for (A) RANKL, (B) OSCAR, (C) TRAP and (D) Cathepsin K. * P<0.05 with comparisons shown by lines. RANKL, receptor activator of nuclear factor-κB ligand; OSCAR, osteoclast associated Ig-like receptor; TRAP, tartrate-resistant acid phosphatase.

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: mRNA levels of the osteoclast-specific factors RANKL, OSCAR, TRAP and Cathepsin K. Expression levels of mRNA are presented for the control and adiponectin-treated group for (A) RANKL, (B) OSCAR, (C) TRAP and (D) Cathepsin K. * P<0.05 with comparisons shown by lines. RANKL, receptor activator of nuclear factor-κB ligand; OSCAR, osteoclast associated Ig-like receptor; TRAP, tartrate-resistant acid phosphatase.

Techniques: Expressing, Control

Adiponectin upregulates the AdipoR1 expression level. AdipoR1 levels in (A) the control group and (B) the adiponectin-treated group. (C) Quantification of the AdipoR1 levels in the two groups (26.21±4.27 vs. 29.86±6.23%). * P<0.05 with comparisons shown by lines. AdipoR1, adiponectin receptor 1.

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: Adiponectin upregulates the AdipoR1 expression level. AdipoR1 levels in (A) the control group and (B) the adiponectin-treated group. (C) Quantification of the AdipoR1 levels in the two groups (26.21±4.27 vs. 29.86±6.23%). * P<0.05 with comparisons shown by lines. AdipoR1, adiponectin receptor 1.

Techniques: Expressing, Control

Phosphorylation levels of the mTOR pathway. (A) Osteoclast precursor was revealed to be CD14-positive. (B) Isotype control of p-mTOR and p-4EBP1 marked with IgG1-PE. (C) p-mTOR levels in the control group, adiponectin-treated group and adiponectin + MHY1485 group. (D) Histogram of the phosphorylation levels of the mTOR pathway. (E) p-4EBP1 levels in the control group, adiponectin-treated group and adiponectin + MHY1485 group. * P<0.05 with comparisons shown by lines. mTOR, mechanistic target of rapamycin kinase; CD14, cluster of differentiation 14; p-, phosphorylated; 4EBP1, eukaryotic translation initiation factor 4E-binding protein; IgG1, immunoglobulin G1; PE, phycoerythrin.

Journal: International Journal of Molecular Medicine

Article Title: Adiponectin inhibits the differentiation and maturation of osteoclasts via the mTOR pathway in multiple myeloma

doi: 10.3892/ijmm.2020.4475

Figure Lengend Snippet: Phosphorylation levels of the mTOR pathway. (A) Osteoclast precursor was revealed to be CD14-positive. (B) Isotype control of p-mTOR and p-4EBP1 marked with IgG1-PE. (C) p-mTOR levels in the control group, adiponectin-treated group and adiponectin + MHY1485 group. (D) Histogram of the phosphorylation levels of the mTOR pathway. (E) p-4EBP1 levels in the control group, adiponectin-treated group and adiponectin + MHY1485 group. * P<0.05 with comparisons shown by lines. mTOR, mechanistic target of rapamycin kinase; CD14, cluster of differentiation 14; p-, phosphorylated; 4EBP1, eukaryotic translation initiation factor 4E-binding protein; IgG1, immunoglobulin G1; PE, phycoerythrin.

Techniques: Phospho-proteomics, Control, Binding Assay

Schedule to develop in vitro cultured human epidermal equivalents (HEEs) model mimicking atopic dermatitis (AD). During the preparation of reconstructed three-dimensional epidermis, the cocktail containing Poly(I:C) and inflammatory cytokines was treated on the 11th day after air-lift to generate AD-HEEs. Adiponectin was co-treated with the cocktail at the same time to investigate the effect of adiponectin on AD conditioned epidermis. And then the samples were harvested for analysis 3 days later. Tx.: treatment, TLR: Toll-like receptor 3, TNF-α: tumor necrosis factor-alpha, IL: interleukin.

Journal: Annals of Dermatology

Article Title: Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis

doi: 10.5021/ad.2019.31.2.186

Figure Lengend Snippet: Schedule to develop in vitro cultured human epidermal equivalents (HEEs) model mimicking atopic dermatitis (AD). During the preparation of reconstructed three-dimensional epidermis, the cocktail containing Poly(I:C) and inflammatory cytokines was treated on the 11th day after air-lift to generate AD-HEEs. Adiponectin was co-treated with the cocktail at the same time to investigate the effect of adiponectin on AD conditioned epidermis. And then the samples were harvested for analysis 3 days later. Tx.: treatment, TLR: Toll-like receptor 3, TNF-α: tumor necrosis factor-alpha, IL: interleukin.

Article Snippet: Recombinant human full-length adiponectin protein was obtained from Biobud (Seongnam, Korea).

Techniques: In Vitro, Cell Culture

Immunohistochemical staining of normal skin and atopic dermatitis lesional skin for adiponectin receptors. Protein expression of (A) adiponectin receptor-1 (AdipoR1) and (B) -2 (AdipoR2) in human epidermis were diminished in 3 atopic dermatitis patients, compared to control epidermis from 3 normal young adults (n=3 in each group). Bar=100 µm.

Journal: Annals of Dermatology

Article Title: Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis

doi: 10.5021/ad.2019.31.2.186

Figure Lengend Snippet: Immunohistochemical staining of normal skin and atopic dermatitis lesional skin for adiponectin receptors. Protein expression of (A) adiponectin receptor-1 (AdipoR1) and (B) -2 (AdipoR2) in human epidermis were diminished in 3 atopic dermatitis patients, compared to control epidermis from 3 normal young adults (n=3 in each group). Bar=100 µm.

Article Snippet: Recombinant human full-length adiponectin protein was obtained from Biobud (Seongnam, Korea).

Techniques: Immunohistochemical staining, Staining, Expressing, Control

Effects of adiponectin on inflammatory markers in three-dimensional skin atopic dermatitis (AD) model. The quantitative real-time reverse transcriptase polymerase chain reaction analysis showed the changes in the expression of major inflammatory markers by adiponectin treatment. (A) carbonic anhydrase II (CAII), (B) neuron-specific NEL-like protein 2 (NELL2), (C) thymic stromal lymphopoietin (TSLP) were evaluated as the specific inflammatory markers for atopic dermatitis. Others, (D) human beta-defensin 2 (hBD2), (E) interleukin (IL)-8, and (F) tumor necrosis factor-alpha (TNF-α) were assessed as the primary inflammatory markers. * p <0.05, ** p <0.01.

Journal: Annals of Dermatology

Article Title: Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis

doi: 10.5021/ad.2019.31.2.186

Figure Lengend Snippet: Effects of adiponectin on inflammatory markers in three-dimensional skin atopic dermatitis (AD) model. The quantitative real-time reverse transcriptase polymerase chain reaction analysis showed the changes in the expression of major inflammatory markers by adiponectin treatment. (A) carbonic anhydrase II (CAII), (B) neuron-specific NEL-like protein 2 (NELL2), (C) thymic stromal lymphopoietin (TSLP) were evaluated as the specific inflammatory markers for atopic dermatitis. Others, (D) human beta-defensin 2 (hBD2), (E) interleukin (IL)-8, and (F) tumor necrosis factor-alpha (TNF-α) were assessed as the primary inflammatory markers. * p <0.05, ** p <0.01.

Article Snippet: Recombinant human full-length adiponectin protein was obtained from Biobud (Seongnam, Korea).

Techniques: Reverse Transcription, Polymerase Chain Reaction, Expressing

Representative microscopic finding of (A) normal untreated control, (B) control for reconstructed epidermal atopic dermatitis (AD) model (only the inflammatory cocktail treated), and (C) the adiponectin treated sample (the inflammatory cocktail and adiponectin co-treated). HEEs: human epidermal equivalents, Tx.: treatment.

Journal: Annals of Dermatology

Article Title: Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis

doi: 10.5021/ad.2019.31.2.186

Figure Lengend Snippet: Representative microscopic finding of (A) normal untreated control, (B) control for reconstructed epidermal atopic dermatitis (AD) model (only the inflammatory cocktail treated), and (C) the adiponectin treated sample (the inflammatory cocktail and adiponectin co-treated). HEEs: human epidermal equivalents, Tx.: treatment.

Article Snippet: Recombinant human full-length adiponectin protein was obtained from Biobud (Seongnam, Korea).

Techniques: Control

Effects of adiponectin on expression of lipid synthesis related gene in atopic dermatitis reconstructed epidermal model. By using the quantitative real-time reverse transcriptase polymerase chain reaction analysis, major rate-limiting epidermal lipid biosynthesis enzymes (A) fatty acid synthase, (B) HMG CoA reductase, and (C) serine palimitoyl transferase, and the transcription factors encoding for lipid synthesis enzymes (D) sterol regulatory binding protein 1a (SREBP1a), (E) SREBP1c, and (F) SREBP2 were assessed. * p <0.05, ** p <0.01.

Journal: Annals of Dermatology

Article Title: Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis

doi: 10.5021/ad.2019.31.2.186

Figure Lengend Snippet: Effects of adiponectin on expression of lipid synthesis related gene in atopic dermatitis reconstructed epidermal model. By using the quantitative real-time reverse transcriptase polymerase chain reaction analysis, major rate-limiting epidermal lipid biosynthesis enzymes (A) fatty acid synthase, (B) HMG CoA reductase, and (C) serine palimitoyl transferase, and the transcription factors encoding for lipid synthesis enzymes (D) sterol regulatory binding protein 1a (SREBP1a), (E) SREBP1c, and (F) SREBP2 were assessed. * p <0.05, ** p <0.01.

Article Snippet: Recombinant human full-length adiponectin protein was obtained from Biobud (Seongnam, Korea).

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Binding Assay

Effects of adiponectin on epidermal differentiation in atopic dermatitis (AD) reconstructed epidermal model. The mRNA expression of the major epidermal differentiation markers (A) filaggrin (FLG), (B) loricrin, and (C) involucrin was quantified using real time reverse transcriptase polymerase chain reaction. (D) Representative immunohistochemical staining of untreated normal epidermal equivalent (normal-HEEs), and AD mimicking epidermis equivalent (AD-HEEs), and adiponectin treated AD mimicking equivalent (AD-HEEs+adiponectin) with human filaggrin antibody. Tx.: treatment. Bar=50 µm. * p <0.05, ** p <0.01.

Journal: Annals of Dermatology

Article Title: Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis

doi: 10.5021/ad.2019.31.2.186

Figure Lengend Snippet: Effects of adiponectin on epidermal differentiation in atopic dermatitis (AD) reconstructed epidermal model. The mRNA expression of the major epidermal differentiation markers (A) filaggrin (FLG), (B) loricrin, and (C) involucrin was quantified using real time reverse transcriptase polymerase chain reaction. (D) Representative immunohistochemical staining of untreated normal epidermal equivalent (normal-HEEs), and AD mimicking epidermis equivalent (AD-HEEs), and adiponectin treated AD mimicking equivalent (AD-HEEs+adiponectin) with human filaggrin antibody. Tx.: treatment. Bar=50 µm. * p <0.05, ** p <0.01.

Article Snippet: Recombinant human full-length adiponectin protein was obtained from Biobud (Seongnam, Korea).

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Immunohistochemical staining, Staining

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recombination human full length adiponectin (BioVendor Instruments)

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Colony Assay:

Incubation:

Western Blot:

Cell Culture:

Activation Assay:

Flow Cytometry:

Expressing:

Quantitative RT-PCR:

Transfection:

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